Summary

This notebook demonstrates how to recreate lineages published in the paper Live imaging of remyelination in the adult mouse corpus callosum and available at idr0113-bottes-opcclones.

The lineage is created from the metadata associated to the specified image.

To load the data from the Image Data Resource, we use:

• the Python API
• the JSON API

LPC-induced focal demyelination and in vivo imaging of genetically targeted OPCs and their progeny to describe the cellular dynamics of OPC-mediated remyelination in the CC.

Longitudinal observation of OPCs and their progeny for up to two months reveals functional inter- and intraclonal heterogeneity and provides insights into the cell division capacity and the migration/differentiation dynamics of OPCs and their daughter cells in vivo.

The majority of the clones remained quiescent or divided only few times. Some OPCs were highly proliferative. Large clones showed longer times between consecutive divisions compared to low proliferating clones.

OPCs show distinct modes of cell division: from symmetric proliferative, to symmetric differentiating and also asymmetric cell division, where the OPC is self-renewed while the other daughter cell differentiates.

Only 16.46% of OPC-derived cells differentiated into mature, remyelinating oligodendrocytes, with OPCs born at early divisions showing a higher probability to survive and to terminally differentiate.

Cell death was associated with distinct cell division histories of different clones, with higher probability of death when generated at later divisions.

Migratory behaviour was restricted to progenitors. Successfully differentiating progenitors moved shorter distances per day compared to dying cells.

Inputs

Parameters needed to configure the workflow:

imageId: Identifier of an image in IDR.

Ouputs

Output file generated:

lineage_imageId.pdf: A PDF with the generated lineage. Options to save as png or svg are also available.